API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family. Changes to thesaurus. Changes to database old new. Taxons. Notes. Identification. Additional tests. API® 20 E v v X. X. X. X. RapiD 20 E™ v v X. API® 20 Microbial Identification Kits, bioMerieux. Supplier: Quantity. API® 20 E Microbial Identification Kit, , , , Pack of 25, Retrieving.
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Identification of Pseudomonas aeruginosa with the API-20E system.
Reactions obtained using the Microbact System may differ from published results using other substrate formulations. Used materials should be autoclaved, incinerated, or immersed in germicide before disposal.
A small amount of zinc powder should be added to those wells which exhibit a yellow colour after the addition of the nitrate reagents. The 12A 12E strip should be read at hours. Reading the test strip 1. Thomas, Veterinary Microbiology. Identification of Bacteria by Computer: Motility and DNase tests are recommended for further speciation of this group. Citrate is the sole carbon source, which if utilized results in a pH rise, indicated by bromothymol blue, with a colour change from green to blue.
H 2 S reacts with ferric salts in the medium to form a black precipitate. The following organisms are recommended for independent laboratory assessment. These can be purchased individually or as a set by ordering product code MB This is a negative reaction. Once the foil pouch has been opened, unused strips must be placed back in the foil pouch, and the foil pouch taped closed.
Solid gelatin particles which may drift across the well after rehydration should be considered as a negative reaction. The results should be interpreted as follows:.
Inoculation The wells of individual substrate sets can be exposed by cutting the end tag of the sealing strip and slowly peeling it back. Indole is formed from metabolism of tryptophan. If further speciation is required for Yersinia spp.
For aid in interpreting reactions, refer to the Table of Reactions. Yellow-green is indicative of a negative result. To determine the purity of the inoculum, it is advisable to inoculate a solid non-selective medium with the test suspension to act as a culture purity check. All systems should be read after 48 hours for the identification of Miscellaneous Gram-negative bacilli. Materials Required but not provided The following materials may be required but are not provided: Indole positive organisms may produce a brown colour.
The arginine reaction well 12 for 12B and well 24 gaalerie 24E is interpreted differently at 24 hours and 48 hours incubation. Hydrolysis of gelatin is indicated by dispersal of the black particles throughout the well. Qualified Personnel should collect specimens according to standards zpi required for specimen handling Production of indolepyruvate by deamination of tryptophan.
Utilisation of Na malonate at the same apo that ammonium sulphate is utilised as the nitrogen source apo sodium hydroxide resulting in increased alkalinity and a blue colouration.
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A Gram-stain and oxidase test should be performed prior to set-up of tests. Warning and precautions 1. Bromothymol blue galerid formation of the specific amine cadaverine. Twelve substrates provide insufficient data to speciate within this group as a single aberrant reaction may result in an incorrect identification.
Argine galeie converts arginine into ornithine, ammonia and carbon dioxide. Green should be regarded as a negative reaction. Test can be evaluated immediately after the addition of the reagent. Strips stored in this manner must be used within 7 days. All organisms belonging to the family Enterobacteriaceae reduce nitrates to nitrites and give a positive reaction.
Apk the results obtained, the indices of the positive reactions are circled. When Actinobacillus or Pasteurella spp. Indole Kovacs reagent forms a pink-red complex with indole. The inclusion of a 12B strip is strongly advised. The results of tests for oxidase, nitrate reduction and motility are included as part of the reaction pattern. Bromothymol blue indicator changes from blue to yellow when the carbohydrate is fermented.
The lysine and ornithine decarboxylase reactions should be carefully interpreted. Incubation Reseal the inoculated rows with the adhesive seal apu write the specimen identification number on the end tag with a marker pen.
The sum of these indices in each group of three reactions forms the code number. Well 8 Indole production – add 2 drops of Indole Kovacs reagent. Some bacterial strains may have atypical biochemical reactions due to unusual nutritional requirements gqlerie mutations and may be difficult to identify. Miscellaneous Gram-negative bacilli – Weakly positive reactions are recorded as negative results.
Mix thoroughly to prepare a homogeneous suspension. Evaluate within 2 minutes of the addition of the reagent. The reactions are evaluated as positive or negative by comparing them with the colour chart. Galedie utilization citrate is the only source of carbon.
The pH shift indicated by bromothymol blue caused by formation of the specific amine putrescine is greater than that caused by lysine decarboxylation.